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Here we describe a complementary approach using an innovative embryonic gut culture system in which the tubular architecture of the gut is maintained and rapid analysis of gene function can be achieved by introduction of expression constructs into the epithelial cell layer by using low-voltage square-wave electroporation.
Complimented strains of MN101 (JR32 lpg0634::aphA-3) and MN102 (JR32 lpg0901::aphA-3) were constructed by introduction of expression vector producing N-terminal M45 epitope tagged-Lpg0634 and Lpg0901, respectively.
Activation of EGF signaling pathways by the introduction of expression of transforming growth factor-alpha (TGFα) through a mammary epithelial cell-targeted transgene can interrupt the downregulation of STAT5 activation and promote mammary epithelial cell survival during involution [ 70].
Similarly, the ARE luciferase activity was increased by the introduction of expression vectors of Nrf2 or Jdp2 in WT MEFs and Jdp2 KO MEFs; however, the MafK repressed the ARE luciferase activity (Supplementary Figures S2c and d).
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Since the introduction of gene expression microarrays in the mid-1990s, genome-wide exprofilingprofiling has been widely utilized in cancer research [63], [64].
Many gene expression signatures were developed since the introduction of gene expression microarray technology, however, so far only in breast cancer two prognostic gene profiles are tested in large prospective trials (Bogaerts et al, 2006; Sparano, 2006; Wirapati et al, 2008; Weigelt et al, 2010).
The adoption of real-time qRT-PCR methodology is somewhat reminiscent of the introduction of cDNA expression microarrays in that initial microarray studies did not identify differentially expressed genes by a statistical method, but by an arbitrary cut-off value of fold-change [ 12].
Let's assume (speculatively but not unreasonably) that Bernanke's introduction of this expression to steer the debate about this issue was intentional.
The introduction of prokaryotic expression elements and the precise design of the compatible RBS and Kozak sequence endow pOmni with pro/eukaryotic dual-expression capability, which was validated by cloning and expression of reporter gene EGFP in E. coli and HeLa cells in this study.
It is known that through the introduction of an expression vector into E. coli or other hosts for recombinant protein production, the native cell functions at many levels can be perturbed (e.g. ribosome functions, RNA turnover as well as energy and intermediary metabolism of the cell) (Bailey 1993; Hoffmann et al. 2002; Lin-Chao et al. 2006).
Introduction of exogenous expression of the rovA gene under the control of the arabinose-inducible promoter araBADp into the mutant strain restored RovA expression when induced with arabinose.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com