The introduction of a constructed adenovirus into the cochlea reportedly results in the successful expression of its contained gene in the guinea pig cochlea without physiological inner ear dysfunction [ 4, 21].
As the transcription activation machinery in yeast is highly conserved, the construction of yeast-based bioassays for hormonal activities only requires the introduction of a receptor construct and a reporter construct.
As shown in Figure 3A, introduction of a twist construct induced expression of the twist protein.
The third and final step in the GEMM-ESC approach is the introduction of a transgenic construct in the Col1a1-frt locus using the Flp recombinase.
Moreover, introduction of a p35S OSR2 construct into bri1-6 and bin2 could partially restore leaf and petiole growth, and the expansion defect in bri1-6 and bin2 leaf cells was partially restored by overexpression of OSR2 (Additional file 1: Figure S5C-F).
Specificity was indicated by successful partial rescue following introduction of an expression construct of C3 resistant to the shRNA (Fig. 3d,g).
Some of the extension proposed and presented cover the aspects of modularization of the specification, the introduction of an iterative construct and a first move to object paradigma.
Therefore, a transient introduction of the construct can lead to a persistent modulation of gene expression without any genomic damage being introduced, hence making epigenetic editing safer and more suitable for therapeutic use.
Genetic modifications of plants (and other organisms) involve the stable introduction of a novel genetic construct (usually carried by a vector) into a target organism's genome.
