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In Canada, tepary bean introduction lines PI319443 and PI440795 have provided the major sources of resistance to CBB in different bean breeding programs.
For example, E28 is a representative line derived from crossing the landrace Ludahonggu with modified introduction lines according its pedigree [ 19].
These sources include the common bean cultivar Montana No. 5 and introduction line PI207262, tepary bean (Phaseolus acutifolius L). introduction lines (PI319443 and PI440795), and scarlet runner bean (Phaseolus coccineus L). [ 3].
To test the hypothesis that the newly identified mutations in FAD2-1B are causative for the elevated oleic acid level in the plant introduction lines, an analysis of the oleic acid phenotype and FAD2-1B genotype association was examined for Population 1, an F6 recombinant inbred line (RIL) population developed from the cross 'Jake'[ 31] × PI 283327.
Genomic DNA of 16 pea genotypes including widely grown cultivars and plant introduction lines (i.e., Shawnee, Melrose, Medora, Lifter, Radley, PI 179449, Green Arrow, Frolic, A778-26-6, Sparkle, JI73, Bohatyr, ICI12043, PI 240515, PI 103709, PI 169603) was extracted from leaf tissue using a modified cetyltrimethylammonium bromide (CTAB) extraction protocol (Rogers and Bendich, 1985).
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Introduction Line 13 (minor): indicate which hereditary disease (colon cancer?).
But the first mention is in 'Introduction' (line 124).
Perhaps biological conditions too? 2. Introduction, line 4.
Minor suggestions: In the paragraph of LA function, the introduction line needs a reference.
Corresponding changes in the manuscript are highlighted in grey. 1. Introduction, line 2. "physical or chemical conditions".
I have only a very minor concern: in the Introduction (line 8) Plasmodium malaria should be re-written as Plasmodium malariae.
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