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To copy them without introducing the length and abundance bias which is characteristic of conventional PCR, we developed an efficient extraction procedure (Figure S1A) and a linear PCR procedure ("ds-Linear PCR") (Figure 1B, S1B, S2) which in a final step generates double-stranded products.
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In order to quantitatively measure the increase in distances from the metabolite under study to the rest of the metabolic network when enzyme j is knocked out, we introduce the length increasing parameter (∆L j ), which essentially averages distance differences between the knockout and wild-type scenarios (see Methods section).
We first describe normalized statistical potential terms and introduce the length-corrected QMEAN scores.
Vin can be estimated by introducing the characteristic length of nanoconfinement δ, which is defined as the distance where thermal agitation of water is still significantly influenced by van der Waals Uvdw and Coulomb Uc solid-liquid interactions, namely where kinetic energy of water k B T equals solid-liquid effective potential Ueff=Uvdw+Uc.
Finally it is observed that by considering the size effect and introducing the material length scale parameters, the frequency of the sandwich plate increases.
The high density of read start positions offered by next-generation sequencing data makes it easier to consider a contig coverage measurement as a sum of independent measurements, thus introducing the contig length as an added parameter (cf. Methods).
Complementation experiments by introducing the full length ORF of the gfo gene into the Z. mobilis disruptant strain rescued the growth defect under heat, ethanol, and osmotic stresses (Table 4).
For immunostaining and Western blot analyses, a FLAG-tagged PrPC expression vector was constructed by introducing the full-length b PRNP into the vector pFLAG-CMV-5 (Sigma-Aldrich, St . Louis MO) between the Bgl II and Sal I sites.
Introducing the CDS lengths as a parameter was a good way to simulate the GPI-anchor pathway-mutant screening that was based on the functional complementation assay using candidate gene-expression plasmids.
After introducing in the length features set, we obtained an F-measure of 0.95, and, finally together with the word type features, we achieved the highest performance of 0.97 for the training set (Table 4).
We then introduce the characteristic length to scale the eigencompliances and compliance matrices to compare translational compliances with rotational ones.
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