For immunostaining and Western blot analyses, a FLAG-tagged PrPC expression vector was constructed by introducing the full-length b PRNP into the vector pFLAG-CMV-5 (Sigma-Aldrich, St . Louis MO) between the Bgl II and Sal I sites.
The study first introduces the full nonlinear model developed in Autosim which has 12 degrees of freedom.
Instead, close the quotation at the end of the fragment and introduce the full sentences as a separate, further quotation, preferably as a new paragraph.
"It would seem that Britain and British overseas territories are facilitating alleged corrupt practices by refusing to introduce the full transparency the Conservatives promised but failed to deliver.
After the schoolwide assembly to introduce the full program last September, one frantic parent called to report that her child was refusing to eat anything in the house that was not healthy.
Initially known as the Astronautics and Space Technology Division, the unit operated from day one as an independent academic department, successfully introduced the full set of degrees in Astronautical Engineering, and was formally renamed the Department of Astronautical Engineering in 2010.
Goldberger, a Pulitzer Prize-winning architecture critic, recounts the trade center's emergence in Lower Manhattan; describes three years of proposals and counterproposals for rebuilding the 16-acre site; and introduces the full cast of politicians, developers, architects and city planners competing to shape "the most challenging urban-design problem of the 21st century".
The general relationship between events and durations serves not only to introduce the full demographic time framework but also to compare it with other relatively complicated temporal designs in the literature.
I have introduced the full 'start-over' ERA in each Congress since I arrived because the rights of women deserve to be constitutional.
We therefore introduced the full 5-day ETAT+ training for all final year (year 6) medical students during their paediatric attachment between November 2011 and May 2012.
