The retention of the intronic sequences introduces termination codons within the mature FIBP mRNA leading to premature termination of translation.
Library diversity is further restricted by NNK and NNN saturations which randomly introduce termination codons that may lead to non-functional proteins, possibly leading to aggregation.
C-terminal truncations were created by introducing a termination codon at the appropriate position in the sequence, again using QuikChange™.
Instead, due to the nature of CRNs, a new type of forced call termination is introduced: forced termination due to intra-cellular handoff failure triggered by the arrival of a PU.
Furthermore, we produced an extension domain that corresponds to residues 1 143 of the modified α′ subunit containing four RPLKPW sequences by introducing a termination codon.
The endogenous rev locus was deleted by mutating the start codon (ATG) to ACG and introducing a termination codon at residue 23.
SLC7A3 is highly intolerant to variation in humans, as shown by the absence of variants introducing premature termination codons in ~61.000 subjects of the ExAc database (http://exac.broadinstitute.org/gene/ENSG00000165349) and the absence of deletion encompassing SLC7A3 in the DGV database.
They introduce a termination criterion and terminating algorithms for constraint simplification and improvement.
Polymorphisms not found in cis-regulatory regions can also result in apparent allele-specific splicing if they introduce premature termination codons (PTC) [ 45] and cause differential nonsense-mediated decay of alternative alleles.
In addition, 125 SNPs introduced premature termination codons and 49 removed stop codons.
Most of the deleterious germline mutations in BRCA2 introduce premature termination codons and trigger nonsense-mediated mRNA decay [ 11].
