We introduce gene expression data to filter the interacting neighbors of a protein in order to enhance the degree of functional consensus among the neighbors.
To further evaluate the detailed distribution of the introduced gene expression, immunohistochemical staining for luciferase was performed with an anti-luciferase antibody (more than 3 times for each specimen of nine rats).
In 1999, Golub et al. introduced gene expression profiling as an approach to validate the outcome of clinical diagnosis [ 21].
During the first 2 wk, the biologist instructor introduced gene expression studies, microarray technology and platforms, and the biological aspects of microarrays, and conducted a wet lab microarray experiment with the students.
Next, we introduced gene expression cassettes for Coi12p-ΔFKBD, the Coi12p mutant lacking two FKBDs (Fig 6A, ΔFKBD), and for Coi12p-ΔTPR, which lacked the TPR domain (Fig 6A, ΔTPR).
The Connectivity Map was recently introduced to compare gene expression data by introducing gene-expression signatures (represented by a set of genes with up- or down-regulated labels according to their biological states) and is available as a web tool for detecting similar gene-expression signatures from a limited data set (approximately 7,000 expression profiles representing 1,309 compounds).
Two seminal studies [ 30, 31] introduced gene expression-based classifiers to distinguish cases of BL and DLBCL based on data sets from different array platforms.
To confirm the successful expression of introduced gene products in OSE cells, the expressions of LT, c-erbB-2 and mutant Ha-ras protein were confirmed by Western blotting, as shown in Figure 1A, B and C, respectively.
As a reporter gene for evaluating and optimizing the protocol for transient expression of the introduced gene in planta, we monitored transient expression of β-glucuronidase (GUS) in alfalfa leaves.
hEPO levels were much higher (∼30-fold) than GFP, indicating plasmid-based introduced gene copies have higher expression than baculovirus-based introduced genes.
It is often difficult to obtain transgenic animals by the classical pronuclear injection method, [ 10] and molecular characterization of the introduced gene (genotyping) and its expression must often be performed.
