Exact(2)
First of all, this is the first study demonstrating that direct lineage-specific conversion to NPCs can be achieved by chemical cocktails without introducing expression of exogenous genes.
To circumvent this problem, we engineered S. cerevisiae to produce LA and ALA by sequentially introducing expression cassettes containing cDNAs encoding LuFAD2 [ 30] and LuFAD3b [ 28].
Similar(58)
We introduced expression plasmids carrying dptD or NRPS genes encoding subunits from two related lipopeptide biosynthetic pathways into a daptomycin nonproducing strain of S. roseosporus harboring a deletion of dptD.
Microprojectile bombardment has been used as an alternative delivery method to introduce expression construct in cereal leaf cells.
In summary, we have developed a simple and efficient DNA delivery method to introduce expression vectors into embryos and F0 progenies.
The difficulties arise on the one hand from randomly introduced expression cassettes leading to unpredictable gene copy numbers and different transcription efficiencies influenced by different surrounding chromosomal environments (position effect).
Our previous studies have shown that introduced expression of BRAF V600E in thyroid cells could induce the silencing of various thyroid iodide-handling genes, most prominently the NIS gene (Liu et al. 2007).
Almost all of the crafts practiced in prehistoric times are still practiced today, along with some newly introduced expressions.
Although these approaches are adequate for simple genetic manipulations, such as introducing small expression cassettes, they have several limitations for more sophisticated synthetic biology applications.
Dataset normalization is a widely adopted solution to such errors, however this may not completely prevent introducing incorrect expression ratios.
Strawson made no mention of Locke, Aristotle or Frege in the context of introducing the expression 'sortal universal' in Individuals in 1959.
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