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A double mutation was achieved using the mutated Cys286 DNA as a template for introducing a mutation at Cys295 to alanine.
Other targeted modifications include placing genes under the control of heterologous regulatable or constitutive promoters, introducing epitope/protein tags to the target protein, or introducing a mutation into the target gene.
Indeed, it was found that inhibiting GABAB1 GABAB1 interactions using either a non-functional GABAB1 subunit as competitor or introducing a mutation in the GABAB1 VFT domain increased signalling efficacy by approximately 50% (Maurel et al. 2008; Comps-Agrar et al. 2011).
The mutant forward primer is designed across the first miR-130a binding site, introducing a mutation that scrambles the entire seed region (underlined portion, see Fig. 4A): mutant3′UTR mutant primers: forward, 5′-GGCCAGAAGTAGCTAACGTGATTTCTA AACTAGGCTC-3′; and reverse, 5′-GCCTAGTTTAGAAATCACGTTAGCTACTTCTGGCCC-3′.
First we start by introducing a mutation in the operator controlling the expression of Tet-ON.
Thr189 is usually autophosphorylated, but this modification can be abrogated by introducing a mutation at this site [9].
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Authors in [36] introduced a mutation operator guided by preferred regions to enhance the performance of an existing set-based evolutionary many-objective optimization algorithm.
The work also introduced a mutation process to use the operators as a test criterion, for evaluation and selection of test cases.
In unpublished results, scientists used gene-editing technology to introduce a mutation in the myostatin gene in pig fetal cells that causes muscle cells to multiply.
To detect such a hostile application we introduce a mutation analysis that checks the ability of an application to be malicious.
Currently, in an infectious clone, it is possible to introduce a mutation on the viral genome at a particular position, or to process recombination between two strains of a particular virus.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com