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We first introduced terminal alkynes instead of olefins to overcome the limited substrate scope of pyridine‐based dipoles and facilitated the 1,3‐dipolar cycloaddition of the terminal alkyne with the resulting azomethine ylide by the use of a coinage‐metal catalyst.
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PEG was conjugated with tyramine to introduce terminal phenolic hydroxyl (Ph-OH) groups.
Two years ago, Montana started a program to introduce terminals at 42 farmers' markets; in Iowa, the state gives wireless machines to farmers and pays all monthly fees associated with them.
In TTAS method, PCR anchor sequences are introduced by terminal elongation instead of ligation, which has been known to suffer from unsatisfied reaction efficiency (Cao et al., 2017).
Each primer introduced a terminal NheI site.
In NCL, a native peptide bond is formed between one polypeptide bearing a chemically introduced C-terminal thioester and a second polypeptide containing an N-terminal cysteine residue.
Synthesis and purification of recombinant Staphylococcal protein A with specially introduced C-terminal cysteine residue (SPA-Cys) were described in [25].
The introduced C-terminal cysteine residue mediates spontaneous peptide dimerization that can be further facilitated by enhanced oxygenation generated by stirring.
The cloning procedure introduced C-terminal c-myc and His6 tags in the protein.
Thereby, a myc tag is introduced N-terminal to the KKEK (Lys-Lys-Glu-Lys) ER retention signal.
For PEG-KR21 synthesis, a 19-atom PEG linker N-Fmoc- N″-succinyl-4,7,10-trioxa-1,13-tridecanediamine (Sigma) was introduced N-terminal to the sequence of KR21 (Table 1).
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