Exact(1)
The PCR product was digested with StuI and XhoI and was cloned between the corresponding sites in a plasmid similar to the one used as template, in which the StuI site was introduced silently (without changing the protein sequence) upstream to the 1b derived NS5A/B sequence, generating plasmid "pET28a PE QQ delta -DTA (1 187) (instead domain III -full 2a JFH1 NS5AB-15aa lIII -full-HIS-KDEL".
Similar(59)
By contrast, Mr. Stone's first expert, Stewart Brand, the founder and publisher of the "Whole Earth Catalog," is introduced looking silently over a pristine stretch of coast.
In addition to a baseline condition (DG), a third-party anonymous subject was introduced who either silently observed or observed and got to give feedback by choosing one of seven messages consisting of varying degree of (dis)satisfaction.
The company silently introduced a trade-off between battery life and performance reduction without telling iPhone owners a $79 replacement battery (third parties charge less) would restore both.
Calder introduces the figures silently one by one, manipulating them and activating the low-tech mechanisms (cranks, pull-strings, air hoses) that animate their activities.
Forbes Ewan Spencee has written about this, and Apple already faces almost 60 Class Action lawsuits in the US and legal action from multiple governments around the world for silently introducing what many deem is 'planned obsolescence' and Italy has already issued fines.
Sitting comfortably and with eyes closed, settle briefly and silently introduce the sacred word as the symbol of your consent to God's presence and action within.
Teddy Kennedy decided to endorse Obama in part because he was upset that Hillary sat silently when Francine Torge introduced the New York senator at a New Hampshire event saying: "Some people compare one of the other candidates to John F. Kennedy.
In a drafty classroom downstairs, I was introduced to members of the faculty, who sat silently and warily, arms folded, as, with the help of an interpreter, I fumbled through an explanation of my interest in new Romanian film.
The woman who lived there refused to open the door, and she stood silently on the other side while Leslie introduced herself.
As for the supporters, many speak virtually no English but still sit for hours silently observing the trial while prosecutors have introduced photos and X-rays of the dead baby.
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Justyna Jupowicz-Kozak
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