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Gladysz has contributed to books, organized exhibits, appeared on television and radio, and introduced silent films all over the world.
We introduced silent mutations of 3 codons within the shRNA target common sequence.
In addition, we introduced silent mutations and an N-terminal flag tag into a 1 220 residue LAMR construct previously used to generate a crystal structure.
To explore this possibility, we introduced silent mutations in the cat gene upstream of the synonymous coding region of ppa in the mutation cassette to generate a ribosome binding site more closely resembling the bacterial consensus ribosome binding site [ 20].
Kudla et al. (2009) clearly showed the effect of codon bias by synthesizing and expressing 154 genes encoding the green fluorescent protein (GFP) with randomly introduced silent mutations in the third base position.
For example, in K747_03825, the 12-bp repeat sequence GGGGGGGGGGGG was changed to GGAGGAGGCGG, which simultaneously introduced silent mutations to prevent replication slippage and shortened the length to 11, bringing S2 in frame with S1.
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· T in the Park has followed Glastonbury's example by introducing "silent discos".
In our studies, we used this technology to destabilize the predicted hairpin by introducing silent mutations within the first 72 nucleotides of the coding sequence.
In this study, the effect of rare codons on hG-CSF expression level was evaluated through introducing silent mutations in the 5′-end of the coding sequence.
This reading introduces silent appeal to a principle either absolute or contributory, according to one's way of understanding 'that is wrong'.
By introducing silent mutations, we increased the soluble expression level in E. coli by 2000-fold without altering the CVN protein sequence, and the specific activity was slightly higher for the optimized CVN than for the wild-type variant.
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