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The introduced mutation was retained, and thus rNiV(W−) was not reverted in the infected animals.
Thus, the introduced mutation promoted more intensive viral uptake in the mouse respiratory tract.
Plasmids were transformed into E. coli DH5α and re-extracted from transformants to confirm the introduced mutation by DNA sequencing.
After transformation in E. coli, those plasmids were again purifed and verified for the integrity of the introduced mutation.
The introduced mutation had a dramatic inhibitory effect on mTORC1 activity (Figure 4A) and lead to a lack of muscle differentiation (Figure S2).
This led us to investigate if the introduced mutation in the CCTα coding region gene was still present in these cells.
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Stability promotes mutational robustness, as newly introduced mutations are often destabilizing in nature.
Rather than study starving people, they introduced mutations into yeast and then observed which strains could no longer survive without food.
Dr. Wimmer said he thought that was because his team deliberately introduced mutations into it to distinguish it from the natural virus.
The introduced mutations were validated in-house and in 44 laboratories using various techniques, including next-generation sequencing.
The introduced mutations not only lead to an improved capability to reduce progesterone but also to altered substrate preference.
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