Sentence examples for introduced into the expression from inspiring English sources
Mutations of the D. rerio constructs were introduced into the expression vectors by site-directed mutagenesis.
The pET24d:: vraF construct was introduced into the expression host E. coli BL21 DE3) by heat shock.
Corresponding KRAS mutations were introduced into the expression vector using QuickChange® II Site-Directed Mutagenesis Kit according to the manufacturer's recommendations (Stratagene).
The 1716 bp fragment (GenBank accession number: JQ080910) was introduced into the expression vector pcDNATM3.1/V5-His® TOPO (Invitrogen) to generate the psurGasp1 vector.
The amplified PCR product was digested with BamHI and EcoRI and introduced into the expression vector pRSETA (Invitrogen) at the BamHI and EcoRI sites.
The amplified DNA was introduced into the expression vector pRSET (UCB) at the NdeI and BamHI restriction sites by standard methods, and the resulting plasmid was transformed into E. coli BL21 DE3) pLysS.
A size dependent collision efficiency was introduced into the aggregation expression and this improved the fitting of the model with the experimental data.
First a global intensity similarity is introduced into the WSF expression.
Mutations predicted to specifically disrupt inter-helical contacts or tertiary structure of ERev were introduced into the Rev expression vector pcH21SL using PCR-based site-directed mutagenesis25.
Further, the introduction of controlled changes completely eliminates the problem of non-linearity of probe effects because the changes are introduced into the final expression levels as if they were measured in the linear region of the dose/response dependence curve.
Complete sequences for ubc-13 were introduced into the GST expression vector pGEX-GW (a gift from Barth Grant), and either GST alone or GST::UBC-13 were expressed in E. coli strain BL21 and purified using glutathione-Sepharose as described previously [97].
