Sentence examples for introduced in frame from inspiring English sources

Furthermore, a short DNA fragment encoding biotinylated domain (BSP) was introduced in frame at the 3' of the P65-β-Fos cassette for biotinylation of Mamu-DR molecules to assemble tetramer (Fig. 2a).

The FLAG tag coding sequence DYKDDDDK, flanked in each side by one A residue was introduced in frame by PCR within the Coiled coding region after the E25 residue, using specifically designed oligonucleotides and the LD16147 (DRGC) coiled cDNA as template.

To facilitate the production of HA-tagged NHP PARs, a ClaI restriction site was introduced, in frame, upstream of the HA-tag sequence in the pcDNA3-huPAR2HA (pcDNA3/huPAR-2ClaHA) by PCR-based mutagenesis using complementary primers C11 C12, in association with primers C3 C13 (Table S1) as previously described [42].

Gal4FF was introduced in frame with exon 2 of evx1 [ 16].

egfp was introduced in frame upstream of the zebrafish smad2 coding sequence.

For generation of the Axin2-lacZ mouse [ 24], the β-galactosidase (NLS-lacZ) gene was introduced in frame to the endogenous Axin2 promoter by homologous recombination, thereby replacing most of exon 2 (MGI Ref ID J 74286) but leaving the Axin2 promoter intact.

These constructs also each contain an artificially introduced in-frame ATG codon at, or upstream or downstream of the GGC1274-1276 position.

Restriction sites were introduced in-frame to the Forkhead and E4BP4 open reading frames (ORFs) to facilitate cloning into the pET-30a expression vector.

The same 22-nt miR-125b miRE as in A was introduced in-frame into different positions before or after a PTC of a modified firefly luciferase reporter gene to obtain a series of miRE-containing plasmids.

For example, the classic Tandem Affinity Purification or TAP tag is a small specific polypeptidic sequence that is introduced in-frame into the C-terminus of a desired protein (bait).

And they don't introduce in-frame stop codons.