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We introduced expression plasmids carrying dptD or NRPS genes encoding subunits from two related lipopeptide biosynthetic pathways into a daptomycin nonproducing strain of S. roseosporus harboring a deletion of dptD.
The difficulties arise on the one hand from randomly introduced expression cassettes leading to unpredictable gene copy numbers and different transcription efficiencies influenced by different surrounding chromosomal environments (position effect).
Our previous studies have shown that introduced expression of BRAF V600E in thyroid cells could induce the silencing of various thyroid iodide-handling genes, most prominently the NIS gene (Liu et al. 2007).
Production of 13R-MO was stable in the engineered strains even after months of continuous cultivation, which may indicate that using the nrsB promoter might help ease the problem of genetic instability of introduced expression constructs, something that has been reported as a recurring problem.
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Almost all of the crafts practiced in prehistoric times are still practiced today, along with some newly introduced expressions.
Microprojectile bombardment has been used as an alternative delivery method to introduce expression construct in cereal leaf cells.
In summary, we have developed a simple and efficient DNA delivery method to introduce expression vectors into embryos and F0 progenies.
First of all, this is the first study demonstrating that direct lineage-specific conversion to NPCs can be achieved by chemical cocktails without introducing expression of exogenous genes.
To circumvent this problem, we engineered S. cerevisiae to produce LA and ALA by sequentially introducing expression cassettes containing cDNAs encoding LuFAD2 [ 30] and LuFAD3b [ 28].
Test expression and solubility testing Plasmids were introduced into expression hosts via electroporation.
We introduced an expression plasmid containing ahpC into NBRC3832, which grows slower than NH04 at 10°C, and found that expression of AhpC enhanced growth.
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