Sentence examples for introduced downstream from inspiring English sources

Through two times of insertion mutation, the Lac operator and ribosome biding site (RBS) was introduced downstream T7 promoter originally used for sequencing in pcDNA 3.1, and T7 terminator was introduced downstream BGH Poly(A) site so as to realize the controlled expression of cloned gene in E. coli.

Following this step, the full 3' intergenic region between the gene of interest and the endogenous downstream gene (amplified by PCR) was introduced downstream of GFP using 2 sites from the small multi-cloning site present in this location.

Two out-of-frame stop codons and a psoralen photo-crosslinking site were introduced downstream of the open reading frame to enhance fusion formation and reduce the time and cost of fusion construction.

To construct plasmid pTMVΔcpGFP, a multiple cloning site (KpnI/XhoI/EcoRV/NotI) was firstly introduced downstream the stop codon of the CP gene in pTMV to generate a new plasmid, pTMV-KXEN.

First, an AflII site was introduced downstream of DIP13 by replacing the fragment between HindIII and PvuII in PQE30-DIP13 with a PCR product amplified from pQE30-DIP13 using PCR mutagenesis by overlap extension with primers S552, A552, S553, A553, S554, and A554 (Table S1).

To obtain the pCMV-Myc::ELuc (nuc) and pCMV-Myc::FLuc (nuc) constructs, the amplified fragments were ligated into the NocI/NotI site of pCMV/myc/nuc (Invitrogen, Carlsbad, CA) in which a triple nuclear localization signal (NLS) from SV40 large T antigen was introduced downstream of the multiple cloning site, for C-terminal fusion to the luciferases.

Thereby a unique BsmBI site was re-introduced downstream of the elongated Poly-Q encoding region.

This extra partition step may introduce downstream quantitative variation due to the inevitable experimental error.

The BES TAR library was constructed from T. brucei Lister 427 strain clone 221a [45], in which a hygromycin-resistance gene had been previously introduced immediately downstream of the promoter of the active VSG221/427-2 expresiten site and a neomycin-resistance gene downstream of the promoter of the silent VSGVO2 expression site [22].

To prevent the expression of full-length V or W protein, a terminator codon was introduced immediately downstream of each RNA editing site by single nucleotide substitution.

A Bstz171 restriction site was introduced immediately downstream of the 3' loxP site to facilitate southern screening of ES cells.