The primers were designed to introduce a total of 12 single nucleotide mutations into the GLS (See Fig. 3), but targeted wobble positions and thus maintained the wild-type Grk protein sequence (Fig 3).
The two nearly identical primer pairs were designed to fit either a uracil-excision strategy or a very similar WHOPS strategy [ 15], and used to introduce a total of 12 mutations in 5 neighboring codons in the CRE1 sequence.
European Union members states are set to introduce a total embargo of Iranian crude oil purchases in July.
By introducing a total unknown vector consisting of generalized displacements and stresses, the Lagrangian equations are transformed to the Hamiltonian equations which can be solved by using the method of separation of variables.
In the first half hour, the befuddled viewer is introduced to a total of six different plot lines, which, for most of the film, seem to have nothing in common with each other.
North Carolina controversially passed an even broader law in March, and at least 30 similar bills have been introduced in a total of 16 states this year alone.
Each of the faucets was presented randomly to a participant for ten consecutive trials before the next (randomly selected) faucet type was introduced, for a total of up to 50 trials per participant.
In all, 1 μg of the reporter plasmid together with 50 ng of pRL-SV40 were introduced into a total of 1 × 10 32Dcellsells by using Nucleofector device and solution V (Lonza, Cologne, Germany).
