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In the first week of April, police in Brixton introduced a stop and search policy, named Operation Swamp after Margaret Thatcher's 1978 assertion that Britain "might be rather swamped by people of a different culture".
C-terminal deletion mutants were constructed with antisense primers that introduced a stop codon and a Kpn site in lieu of Pro 797.
RIG-I (1 796) was constructed with an antisense primer that introduced a stop codon in place of pro797 and a XhoI site.
Two PCR reactions using two mutagenic primers 6Lstop (5'-CTTTCCAATTAAAACAGCTCGG-3') and 6Rstop (5'-CCGAGCTGTTTTAATTGGAAAG-3') that introduced a stop codon at Y755 and a silent Mse1 restriction site for selection and two outside primers (5R: 5'-GATCTGCACCGGGTAGAAGTTG-3' & 5L: 5'-CAACCAGGATGTGGTGGAGATG-3').
We examined NMJs of Lrp4ECD/ECD mice, in which we introduced a stop codon immediately before the transmembrane segment.
As a control, we introduced a stop codon immediately downstream of the I-SceI site, to generate mHP-I*-RT.
We introduced a stop codon into the last exon of mouse Nrp1, immediately downstream of the transmembrane domain (Fig. 1A).
Most probably, both ORFs were generated as the result of a point mutation that introduced a stop codon in SARI_02719.
This sequence was denoted as a pseudogene, since the indel introduced a stop codon at 7 codons downstream.
To clone graR N, we introduced a stop codon after the 134 th residue using the Quick-Change mutagenesis kit (Agilent, Mississauga, Canada).
He was also carrying a homozygous nonsense mutation in exon 5 in position g.2098C > T that introduced a stop codon at amino acid position 147 (p.Gln147X).
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