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In May, the European commission introduced a restriction on three neonicotinoid-based pesticides – clothianidin, imidacloprid and thiamethoxam – after a string of scientific studies linked neonicotinoid chemicals to harm in bees.
For instance, the Austrian Tax Amendment Act 2014 introduced a restriction on the deductibility of compensation for tax purposes exceeding 500 000 EUR per employee and year.
A third mutagenic primer contained a second substitution that is silent with respect to the protein sequence but which introduced a restriction enzyme recognition site.
Introduction of the hpo mutation introduced a restriction fragment length polymorphism (RFLP) (Tel VR) but it clearly also resulted in loss of methylation at all regions tested, consistent with previous indications that hpo causes a global loss of methylation [ 26].
Four markers required endonuclease digestion of the amplicons (Cleaved Amplified Polymorphic Sequence, CAPS) [ 45], whereas for two markers, the restriction stage had to be preceded by PCR amplification with primers that introduced a restriction site based on a polymorphic locus (derived CAPS, dCAPS) [ 46].
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Danone, the manufacturer of Aptamil and Cow and Gate baby milk powder, said most supermarkets were introducing a restriction of two cans per customer.
Prerequisite to introduce a restriction is an unacceptable risk to human health or the environment which needs to be addressed on a Community-wide basis.
Furthermore, we do not introduce a restriction that the considered signal is narrowband as in [12] since such a restriction is hard to be satisfied in underwater systems [2].
Remark: In CZF only the bounded separation schema introduces a restriction on the complexity of the formulas occurring in it, while all the other schemata allow for arbitrary formulas.
The GarsC201R mutation introduces a restriction site for the enzymes HaeII and HhaI, allowing us to genotype by PCR followed by RFLP analysis.
Using dCAPS, to introduce a restriction enzyme site depending upon the polymophism to allow the SNPs to be defined by polymerase chain amplification followed by restriction enzyme analysis [34], we defined these polymorphisms in the ALS patients and control cases (Figure 1; Tables 1 and 2).
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