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In December 2015, city authorities introduced a point system designed to qualify the cultural status of a building.
The government introduced a point of consumption tax in December 2014 and increased the duty on machine games from 20%to25%5%.
To obliterate this domain, we introduced a point mutation by in vitro mutagenesis to substitute Leu157 to Ala (L157A) in the optineurin sequence.
To ensure that the observed phenotypes were due to inhibition of σE and not overproduction of RseA and RseB, we introduced a point mutation into the rseA gene on the plasmid encoding rseA and rseB, changing the aspartate residue at position 11 of RseA to histidine.
Therefore, in order to evaluate the pathology of this dominant disorder, we have introduced a point mutation into the murine Guca1a gene that causes an E155G amino acid substitution; this is one of the disease-causing mutations found in COD3 patients.
To test this, we introduced a point mutation in the KIF17 motor domain at a conserved residue in the switch II region (KIF17-G234A) that is essential for kinesin motor activity.
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Carey International, based in Washington, introduced a point-to-point service in early October and now serves 70 pairs of cities.
That same year, Peggy introduced a point-of-sale system to allow staff to punch in meal orders on a computer, to streamline operations at the restaurant's various locations.
Australia, Canada and New Zealand – among others – have operated skill-biased migration regimes for many years; the UK has recently introduced a points based system but combined with this with caps on non-EEA migrants.
Groupon introduces a point of sale system for restaurants, and the retailer Urban Outfitters says it will never buy a cash register again.
"It's almost as though they've induced a mass psychosis in society by introducing a point of view that has no common sense and no bearing on any deeper feeling".
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com