To confirm the inhibitory effect of the UL76 ORF translation on the UL77 gene expression, we constructed plasmids with the luciferase gene fused to the C-terminus of the UL 77 ORF and inserted stop codons in the UL76 ORF at 13, 180 and 226 amino acid residues or introduced a frame-shift at 2 amino acid residues, the same as pCMVflag-frame-shift-UL76-77A pCMVflag-frame-shift-UL76-77A pCMVflag-frame-shift-UL76-77A pCMVflag-frame-shift-UL76-77A pCMVflag-frame-shift-UL76-77A
Among several identified amotl2a alleles, two were further analyzed that introduced a frame-shift and a premature STOP codon in the third exon.
Therefore, these mutations do not introduce a frame shift into the coiled coil motif.
In the human genome, the exon 1a′ has been mutated by insertion of two nucleotides introducing a frame shift and resulting in a termination codon.
In all other sequences, excluding O. nerka, a single bp is missing at position 160, which introduces a frame shift and several premature stop codons.
The most influential features describe the translatability of the exon, conservation scores and whether the alternative exon introduces a frame shift.
The BS allele used in this study is eas PC80, which is caused by a 2-bp deletion that introduces a frame shift; the resulting truncated protein lacks a kinase domain and abolishes all enzymatic activity (Pavlidis et al. 1994).
To reconstruct the original phenotype, the plasmid containing the transposon flanked by genomic DNA isolated from 2A01 was reinserted into the genome of PCC 7942 after inactivating the transposase by introducing a frame shift mutation.
Thus, in this paper, we introduce a frame-by-frame intermittent tracking method to shoot non-blurred and bright videos of fast moving scenes with a fixed camera position by alternating the tracking control methods according to whether the shutter is open or closed.
Thus, we introduce a frame-by-frame intermittent tracking method [24] that can reduce motion blur in video shooting by alternating control methods in a high-speed active vision system, from vision-based tracking control to back-to-home control, according to whether the camera shutter is open or closed; the active vision system changes the optical path to the image sensor.
