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These oligos introduced a FLAG tag at the C' terminus of the fusion protein and contained SpeI restriction sites on either end.
To distinguish between the first and the second gene product, we introduced a FLAG tag on the surface portion of the loop 251 266, exactly between the residues 254 and 255 of the first gene product.
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We introduced a FLAG-tagged Klf5 in ESCs and generated two independent pools of stable clones.
To analyze the ability of DRAM to induce apoptosis, we introduced a FLAG-tagged DRAM expression vector into Saos-2Tet-p53-WT or SF126Tet-p53-WT and performed Western blotting and flow cytometry.
Each cDNA of CD8 chimera was subcloned into p3XFLAG-CMV vector (Sigma) to introduce a FLAG epitope tag at the amino terminus.
The pgtA cDNA was excised by digestion with BglII and SpeI (underlined in primer sequences) and ligated into a similarly digested extrachromosomal pDM320 vector (gift of Y. Abu-Kwaik), designed to introduce a FLAG tag at the N-terminus of PgtA (sequence).
By introducing a Flag-tagged NDRG1 expression vector and/or a dominant active β-catenin (the ΔN90 non-degradable mutant, Bahmanyar et al, 2008) into WB1-1, we further established a series of WB1-1 celinesnes (WB1-1/NDRG1, WB1-1/ΔN90-β WB1-1/ΔN90-β WB1-1/ΔN90-β WB1-1/ΔN90-β, referred to as NDRG1, ΔN90, ΔN90/NDRG1).
Clocks changed from Moscow to Bucharest time, and the government introduced a new flag virtually identical to Romania's.
With respect to the old LTE-sim implementation, we introduced a boolean flag, updated every time the user position changes.
We introduced a target flag to nest entrances to elicit defensive responses and quantified four measures of defensivity in 12 stingless bee species in São Paulo State, Brazil.
He has introduced a new state flag, and he flies it all the time.
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