Sentence examples for introduce restriction from inspiring English sources

Primers were used to introduce restriction sites adjacent to the gene.

Sequence changes to this region were generated to introduce restriction enzyme sites used for plasmid construction.

The fragments required were amplified by PCR with primers designed to introduce restriction sites for assembly by standard methods.

PCR-cloning was employed to introduce restriction sites flanking the promoter and transgene to facilitate subsequent cloning.

The pGemT cloned PCR products were reamplified using two oligucleotide primer sets to introduce restriction sites for library cloning.

Successive portions of the bvgAS locus of BPSM were amplified by PCR in order to introduce restriction sites using silent mutations at specific sites corresponding to junctions between structural domains.

Criticizing a number of flaws in Hungarian democracy and governance, Bell noted that "wherever governments introduce restrictions on civil society organizations, to restrict the space for voices that might differ, we do not see a truly free society".

Defra has identified the emerald ash borer as a "significant threat" and worked with other EU nations to introduce restrictions to protect against its spread.

Before the Spanish Senate's vote, some of the country's local authorities had already moved to introduce restrictions on the burqa.

See articlePutting the boot inAfter last summer's "bra wars" over textiles, the European Union appeared set to introduce restrictions on shoe imports from China and Vietnam.

There was no formal agreement between the 20 chairmen and chief executives over how to introduce restrictions.