Undocumented design changes: During implementation, project staff may introduce alterations in project design and intended outcomes that they believe are necessary accommodations to get past unanticipated hurdles or design oversights.
Although mating is a very efficient means of strain improvement and construction of multiple mutants, the fact that sexual development may introduce alterations in the genome has to be considered.
Using molecular dynamic simulation, we showed evidence that this variant has the potential to introduce alterations in the protein or affect RNA splicing.
One possible reason for this discrepancy is that the increased FFA susceptibility would be due to the immortalization process, which may introduce alterations in the lipid metabolism.
Therefore, in this study we introduced two methodological alterations that have been shown to improve children's performance in other tasks with inhibitory demands: (a) imposing a short delay before a child is allowed to act and (b) replacing reward items with symbolic tokens.
The procedure used to create the respiratory-deficient strain S. cerevisiae 311 introduced unknown genetic alterations that could not be directly implemented in the wild-type metabolic network.
Figure 16 shows that all the introduced alterations have an impact on overall performance of the used authentication matching algorithm presented in Section 4. Therefore, we define in Table 5 the quality sets definition for the used matching algorithm.
Unlike transgenic approaches (Placantonakis et al., 2009), this system would ensure reporter expression specifically driven by the endogenous Nestin promoter and/or enhancer, and would not introduce any other genetic alterations that arise from random integration strategies.
Systematically introduced alterations were generated to minimize background expression while maintaining high levels of induced expression.
In this study, we present a comparative analysis of 3D tissue data from ex vivo MRI and whole-heart histological imaging, including quantification of tissue alterations that are introduced by histological processing, as well as of tissue composition.
The formalin introduces crosslinks that can both fragment DNA and cause chemical alterations that may alter sequencing results [ 19].
