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For the RNA binding partner we used the 300-mer 5′ domain of HOTAIR previously established to be necessary and sufficient to bind PRC2 3m, as the size of the full HOTAIR lncRNA (2.2 kb) rendered it intractable to study by EMSA.
Most of these S-secondary symbionts with the primary symbiont inside the bacteriocytes, however, some S-symbionts localize outside the bacteriocytes and suffer occasional horizontal transmission, occur at low titers in hosts within and between species.,,, The remarkable bacterial associations in insects were deemed intractable to study.
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Ever since the first human embryonic stem cells were isolated, scientists have dreamed of using them to study intractable diseases.
The B97-D/TZVP case represents a favorable example with small mean errors and relatively tight error distributions (see Table 1 for other examples), but it would be computationally intractable to use it to study hundreds or thousands of decoys for a system of the size of ubiquitin.
Thus, hESCs provide a unique and powerful system to study otherwise intractable aspects of human development.
The RF1 knockout strain reported here now afford a previously unavailable model organism to study otherwise intractable questions on the codon reassignment process in real time.
In summary, we describe the RNA populations generated by in vitro transcript amplification following 2 methods of cDNA priming and define the limitations of this topical area of bacterial RNA amplification; the successful use of which will enable researchers to study previously intractable host-pathogen interactions where bacterial RNA is limiting.
IMAGINE that the world's best specialists in a particular disease have convened to study a serious and intractable case.
So, we realised that the zebrafish would allow us to study biological functions that are intractable in other animals.
By obtaining the analytical solution for those species of interest, we have provided the means to study systems that might become intractable by other methods.
Thus, zebrafish provide a novel and expedient model system to study this group of devastating, currently intractable, diseases.
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