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To further determine the possible roles of those genes in H2O2 resistance, the sequences of the corresponding genes amplified from wild type mc2155 genomic DNA were cloned into the integrating vector pMV361 (Table S4) and introduced into mc251 via electroporation.
In fact, the whole lux operon was only stably expressed in M. smegmatis when it was cloned into the integrating vector, with no positive clones isolated using the episomal vectors.
For complementation of Δ stf0, the stf0 gene was cloned from Mtb into the integrating mycobacterial expression vector pMV306 under the control of its putative endogenous promoter.
The agl1 gene was amplified from laboratory strain genomic DNA and inserted into the integrating vector pHIS3K-Ptub1 (http://dna.brc.riken.jp/en/yoshidayeast_310en.html, last accessed December 11 , 2015 (Matsuyama et al. 2008).
To confirm that this is the case, we PCR amplified 3.5 kb DNA fragments containing the hsp-nirB-nirD-cobU region of strain 1307/01 (containing the 'C' allele) and the equivalent region from strain 2122/97 (with the 'T' allele) and cloned them separately into the integrating vector pKINT to create the constructs pPG108 and pPG109, respectively.
As trade flows increase and factor inputs imported into the integrating economies begin to circulate freely, production chains crossing the intra-regional national boundaries begin to form.
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Avellino was integrated into the legitimate economy.
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