The PGE based biosensors were mounted into the flow-through cell and calibrated under kinetic regime.
The applicability of the PAO chip to simultaneous online detection of diverse groups of chemicals was demonstrated by the incorporation of a 6-member sensor array into the flow-through chip.
In this system, the sample solution was mixed with the primary Ab and injected into the flow-through biosensor, where it competed with the surface immobilised cortisol for binding to the Ab.
To assess the extent of protein capture by the 5-filtered LeukoCatch system for blood samples, we performed western blot analysis for the proteins retained on the filter (retentate) after PBS wash, and then eluted into the flow-through fraction.
Then, they were incubated with Ni-NTA His·Bind Resin (Novagen), loaded into a column, and the flow-through was discarded.
The supernatants were then applied to ice-cold PBS conditioned MS-columns (Miltenyi Biotec) placed into a magnetic field, and the flow-through material was collected.
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"But what we don't want to see is that any cracks generated at the surface then flow through and into the wheel.
An additional 30 μL of assay buffer were then added to flush the remaining CSF solutions into the same flow-through collection tube.
The gases are injected into the flow reactor through a temperature-controlled, moveable injector.
