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We also discussed the future directions of BAM developments which are gathered around the incorporation of exogenous growth factors into the BAM structure and BAM seeding with cells.
This fragment was inserted into the Bam HI and Sal I sites of p412-GPD to create p412-SSTR2.
The fragments were then inserted into the Bam HI and Sal I sites of pYS0, resulting in plasmids pYS1, pYS2, pYS3, pYS4, pYS5, pYS6, pYS7, pYS8, pYS9, pYS10, pYS11, pYS12, pYS13, pYS15, pYS20, pYS21, pYS22, pYS30, and pYS40.
The fragments were then inserted into the Bam HI and Sal I sites of pYS0-sst, resulting in plasmids pYS-notag3-sst, pYS-notag20-sst, and pYS-notag38-sst.
The PCR fragments of gpps and ls were cloned into the Bam HI Eco RI and Eco RI Sal I sites of the pTrcHis2B vector to create p40Trc-ls-gpps and p40Trc-gpps-ls plasmids.
The BAC clone OSJNBa0014D2 was partially digested by Sau 3AI and cloned into the Bam HI site of binary vector pYLTAC7 (Liu et al. 1999) (provided by RIKEN BioResource Center).
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We rolled into the BAM-spawned city of Severobaikalsk, nestled between the bare rolling hills overlooking Baikal.
The dxs-idi operon was cloned into pTrcHis2B vector by the Bam H I-Kpn I sites to create p40Trc-dxs-idi plasmid.
The C.K2 cassette from the pRL446 plasmid [ 45] was sub-cloned into the blunted Bam HI site of pLY75 to generate the plasmid pLY76.
Because RNA-seq data are increasingly processed into the compact BAM form, we propose that bam2ssj be used as a standard operating procedure for counting splice junction reads.
PCR on cDNA was performed using oligonucleotides ATHB1F and ATHB1R for ATHB1, ATHB1F and ATHB1WCTR for ATHB1WCT, and ATH1CTF and ATHB1R for ATHB1CT (Table 3), and the PCR products cloned into the EcoRI/ Bam HI or the EcoRI/ Sal I sites of the pGBKT7 vector, respectively.
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