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Cells were transformed with plasmids for WT α-syn (pTF201), A30P (pTF202) or A53T (pTF203) and with an Open Biosystems plasmid containing one of the five genes of interest, pre-grown in non-inducing media to mid-log phase, shifted into inducing media for 3 h, and assayed for ROS accumulation using DHR123.
Transformants were pre-grown in non-inducing media to mid-log phase and then shifted into inducing media.
Similar to the experiment shown in Figure 2, cells were pre-grown in non-inducing media until mid-log phase and then shifted into inducing media.
To exploit this phenomenon, cells harboring pTF201 (WT α-syn) and a high copy yeast library were pre-grown in non-inducing media until mid-log phase and then shifted into inducing media and plated on solid agar plates that contained a disk of concentrated hydrogen peroxide.
In brief, yeast cells transformed with plasmids for the various α-syns (pTF201-WT, pTF202-A30P or pTF203-A53T) and the Open Biosystem plasmid harboring the gene of interest were cultured in non-inducing media to mid-log phase and then shifted into inducing media for 3 h at 30°C.
Although the speech was, in format, a typical "laundry list" of ideas and programs, it never devolved into inducing glassy-eyed stares from the audience.
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Fibroblasts with boosted FOXN1 transformed themselves into "induced" TECs (iTECs).
Adult somatic cells (e.g., from blood) from any patient can be reprogrammed into induced pluripotent stem (iPS) cells.
Interestingly, the authors also reported an induction in SIRT1 protein levels when the MEFs were reprogrammed into induced pluripotent stem (iPS) cells.
Somatic cell reprogramming into induced pluripotent stem cells (iPSCs) induces changes in genome architecture reflective of the embryonic stem cell (ESC) state.
Somatic cells can be reprogrammed into induced pluripotent stem (iPS) cells.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com