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Drp1 is located mostly in the cytosol of mammalian cells and a pool of the protein translocates to the mitochondrial tubules where it assembles, through its interaction with hFis1 [5], [5], into foci at future fission sites [6], [7].
Co-localization index for mRNA recruitment into foci (mean and s.e.m., n = 10) (right).
Treatment with transcription inhibitors redistributes GANP from NPCs into foci throughout the nucleus.
As expected, IPA caused relocalization of IRE1α-GFP into foci, consistent with sustained IRE1 activation.
This was further confirmed by the redistribution of 53BP1 staining into foci in some mutant cells (Fig. 6F).
Furthermore, specific proteins can cycle into foci from the cytoplasm in less than a minute (Aizer et al. 2008).
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