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The proposed system showed an improved assay performance due to the introduction of IGQ-structure into fluorescent signaling probes, which could inhibit the background fluorescence and increase fluorescence restoration amplitude of fluoresceins upon target DNA hybridization.
Phycobiliproteins conjugated to immunoglobins, protein A, and avidin were developed into fluorescent probes and have obtained wide usage in histochemistry, fluorescence microscopy, flow cytometry, fluorescence-activated cell sorting, and fluorescence immunoassays [ 2, 95, 96].
The local activity inside catalyst particles can be resolved by monitoring the fluorescence signal originating from the conversion of fluorogenic reactants into fluorescent reaction products.
Enzymes that are able to rapidly convert substrates into fluorescent products and receptors for which ligands are available that show fluorescence enhancement in the binding pocket are most suitable in this regard.
This fluorescence-based assay relies on ROS converting the nonfluorescent DHR reagent into fluorescent rhodamine123b, providing a more quantitative measure of ROS production.
We also measured cell stress by assessing the fraction of cells staining positive upon incubation with dihydroethidine (DHE), which is converted into fluorescent ethidium in ROS-overproducing cells but also gives rise to strong fluorescence in cells that have lost their plasma membrane integrity.
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Fluorogram of normalized fluorescence signal collected from the direct titration of CRM1 into fluorescent-labeled PKINES peptide in presence of excess Sc RanGTP is plotted on the top panel.
Because the nondecalcified samples at 8 weeks were too hard to be made into fluorescent-labeled sections due to high degree of calcification, therefore the samples only at 4 weeks were made into the fluorescence-labeled sections.
The latent fluorophores was rearranged into the fluorescent coumarin derivatives with strong fluorescence by a gold ion-mediated hydroarylation reaction.
DCF-diacetate is cell permeable and is cleaved by esterases and then oxidized into the fluorescent DCF by ROS, thus fluorescence is proportional to intracellular ROS levels.
Lead dioxide solid-phase reactor incorporated into FIA manifold for oxidation of FA into a fluorescent derivative has been employed to determine the analyte in pharmaceuticals [19].
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