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This DNA fragment was isolated from the gel and inserted into a vector for nucleotide sequencing.
This specific fragment was isolated from agarose gel and ligated into a vector for DNA sequencing.
To address this question the C-terminal 123 amino acid residues of Aae MutL, residues 303 425, were cloned into a vector for heterologous expression in E. coli.
A 997-bp deletion was isolated, amplified, and cloned into a vector for use as a control molecule (Fig. 5B).
cDNAs were synthesized (Sigma) and cloned upstream of a GFP tag; they were then subcloned into a vector for transcription, either pSP6-4T or pCS2+.
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Amplification of the DEG7 cDNA with primers 0740 and 0799, using pHS52 as a template, and ligation of the respective DNA into pET151-D/TOPO, created a vector for overexpression of full-length His6-tagged DEG7 in E. coli (pHS166).
To engineer cell lines, primers were designed to amplify cDNA from the pEF6-HA-pTGFβ1(a) vector for cloning into the multicloning site of the pBMN-Ires-eGFP retroviral vector (courtesy of Dr. Gary Nolan).
DNA fragments encoding the identified domains were synthesized (GenScript Inc., Piscataway, NJ, USA) and cloned into a vector designed for the expression and purification of domains fused to the carboxyl terminus of glutathione S-transferase (GST), as described previously [ 57].
Single chain variable fragment (scFv) molecules were selected from a synthetic phage display library then cloned into a generic vector for expression of the scFv fused to the light chain constant domain of human immunoglobulin with a C-terminal cysteine residue (scFvCLcys).
Each of the 37 predicted metagenome-derived cellulase genes were synthesized and cloned into a custom vector for in vitro cell-free expression using a T7 promoter/terminator-based system [ 20].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com