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We propose a mechanism for embelin-induced rapid conversion of PAI-1 into a substrate for its target proteases and the subsequent slow conversion of PAI-1 into an irreversibly inactivated form.
Fusion of Cas9 to the N-terminal region of human Geminin converted this gene-editing protein into a substrate for the E3 ubiquitin ligase complex APC/Cdh1, resulting in a cell-cycle-tailored expression with low levels in G1 but high expression in S/G2/M.
Fusion of the α2AP motif changed HSA into a substrate for transglutamination by fXIIIa.
Therefore phosphorylation at Ser235 turns tau into a substrate for GSK3 at Ser231 making this site look like a CDK5 site in cells.
In the present study, we have demonstrated that the addition of the 21 amino acid C-terminal GPI-T signal sequence of Y21 is sufficient to convert the soluble form of INV into a substrate for GPI-T.
The localization of INV to the plasma membrane and the outer cell wall confirms that the Y21 signal sequence is sufficient to convert INV into a substrate for GPI-T.
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In this case, the primary use that comes into consideration is as a substrate for energy production in biogas plants [3, 5].
The trans-splicing technology uses a trans-splicing molecule that "tricks" the spliceosome into using it as a substrate for splicing.
Through DNA replication or nick-induced gap filling synthesis, the damage-containing hairpin can be reincorporated into duplex DNA, regenerating a substrate for BER.
Alternatively, and/or in addition, some may arise simply because specific regions of the genome are predisposed to generate piRNAs, with whatever is inserted into the locus becoming a substrate for piRNA production.
In the liver, alanine is deaminated into pyruvate, which serves as a substrate for gluconeogenesis.
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