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Cycling conditions were 94°C for 2 min; 35 cycles of 94°C for 30 s, 55°C for 30 s, and 68°C for 7 min 45 s; followed by a final extension at 72°C for 7 min. After PCR amplification, the amplicons were fragmented, converted into a sequencing library, and sequenced by using the Genome Sequencer FLX System (Roche, Copenhagen, Denmark).
Based on a decomposition of the problem into a sequencing and a timetabling problem, we propose two local search algorithms.
Keen to document exactly which microbes are there, many researchers are using brute-force 'metagenomic' studies in which they extract microbial DNA from the body cavity of choice and hurl it all into a sequencing machine.
In this study, synthetic wastewater containing NB, 4-NP, 2,4-DNP, and AN at concentrations ranging from 50 to 180 mg/L was fed into a sequencing batch reactor (SBR).
Poly-A RNA was isolated from ∼1 μg total RNA, and reverse-transcribed into complementary DNA (cDNA) using a random priming cDNA Synthesis kit (Roche).The cDNA was converted into a sequencing library for 454 sequencing using the GS FLX Titanium Rapid Library Preparation kit and sequenced on the Roche 454 platform.
At the time the mid 1990s DNA sequencing was a painstaking affair that involved breaking an organism's genome into pieces, growing the pieces in bacteria or yeast cells, extracting them again, breaking them up still further, feeding the small bits into a sequencing machine, and then trying to put the whole jigsaw puzzle back together in reverse.
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Fingerprints don't fall into a sequence.
The identification problem is decomposed into a sequence of subproblems.
Maazel breaks it into a sequence of sonic blocks - impressive in themselves, but lacking cumulative meaning.
Meanwhile Status Quo's career had evolved into a sequence of special events.
The curve is divided into a sequence of straight line segments of known length.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com