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We also found that the vast majority of signal from the Phsp-16.2 gfp Phsp-16.2 gfpgene oreporterd in transgenetine cells, suggesting that the originatedatterns are caused by inthetintestines.
It was furthermore observed that the phytosterols were found in the collected intestine cells but not transported to the basolateral region in the intestinal cell model system.
In transgenic larvae, GFP was detected in the anterior and posterior intestine cells, vulva muscle, many cells in the head region, and intestinal lumen (Figure S3E H).
At the same time, healthy small intestine cells were used to determine overall toxicity against noncancerous cells.
Moreover, some extent of bioactives escape uptake by small intestine cells, however this procedure is not completely negative as the escaped nutrients may keep the advantageous microflora in colon and thus beneficial compounds are generated via fermentation in bowel.
We measured the in vivo emission spectrum (3 nm resolution) for each fluorescent protein in live C. elegans and showed that we can use those pure spectra to unmix overlapping fluorescent signals in spectral images of intestine cells.
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We have quantified the inhibition of intestinal cholesterol transport by pine-derived phytosterols using an HT29-MTX intestine cell model that forms a mucus layer similar to that in the intestine.
The bacterial adhesion and invasion assays were performed using the human intestine cell line Intestine-407 as previously described [93].
The same solvent mixture has been used for analyte extraction from cell cultures of human lung and intestine cell lines, and also from cell lysates, by 10-fold dilution with 25 μL of culture medium.
From ArrayExpress, we also downloaded a dataset containing the gene expression data for 34 large intestine cell lines hybridized on the Affymetrix HGU133A array (accession number E-MTAB-783 [ 16]).
In the small intestine these cells are enterocytes, paneth cells, goblet cells, and enteroendocrine cells.
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