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The dietary modulation of aspects of fluid homeostasis other than ROD production (namely, fluid content and intestinal emptying rate, Figures 2I and 6A, respectively) suggested additional mechanisms of fluid control.
In sum, this semiautomatic analysis of fly excreta provides an integrated physiological readout for food intake, acid-base balance and diuresis, which reveals dietary modulation of intestinal emptying rate, pH, and fluid content.
In addition to providing quantitative information about intestinal emptying rate, we were able to extract further information about the nature of Drosophila excreta by using the pH indicator dye Bromophenol blue (BPB, Figures 2E 2G) to obtain colored deposits, which we then analyzed semiautomatedly to extract quantitative information about size, shape, concentration, and color.
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This intestinal hormone delays emptying of the stomach, reduces glucagon secretion, and regulates insulin secretion, in fact depending on blood glucose concentrations.
Both intestinal vagus nerve activity and intestinal peptides regulate gastric emptying.
Impaired taste sensation, problems with swallowing, slower gastric emptying and intestinal and colonic transit, and altered levels of satiety hormones have been reported in the elderly [ 6- 10].
Gastric emptying, small intestinal transit time, median velocity of the magnet through the small intestine and contraction patterns were determined using the magnet-based Motility Tracking System-1 (MTS-1) (Motilis, Lausanne, Switzerland) [ 7].
Impaired intestinal/colonic emptying is defined as preserved gastric emptying with depressed intestinal peristalsis and constipation.
Gastric emptying and small intestinal transit were measured as previously described [ 10].
Gastric emptying and small intestinal transit in fasted mice were assessed using gavaged rhodamine dextran.
In health, cholecystokinin (CCK) and peptide YY (PYY) are important humoral mediators of nutrient-induced small intestinal feedback, which regulates gastric emptying (GE) and energy intake [ 1- 5].
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