Sentence examples for intestinal cultures from inspiring English sources
Upper small intestinal cultures were derived from 10 15 cm of intestine immediately distal to the stomach, and colon cultures included tissue distal to the caecum.
Recent advancements in the understanding of the intestinal stem cell niche and primary intestinal culture have enabled long-term primary intestinal cultures and offered promising sources of primary cells and tissue for transplantation in vivo.
We subsequently applied these highly differentiated 3-D intestinal cultures as human surrogate infection models to elucidate the role of all characterized Salmonella T3SSs in the early stages of human enteric salmonellosis.
Mixed intestinal cultures were produced, as previously described [ 11, 14].
In primary murine intestinal cultures, bile acids dose dependently increased GLP-1 release.
Hormone secretion in vitro was measured in GLUTag and primary murine intestinal cultures.
In addition, issues arise when anaerobic pathogens are exposed to the hyperoxic environment required for intestinal culture.
The 3D design reported here allows investigators to setup intestinal culture explants while manipulating the gas media explants are exposed to, to support tissue viability for a minimal of 8h.
FACS-sorted intestinal crypt populations were collected in the intestinal culture medium including Wnt3a (50% conditioned medium) supplemented with the ROCK inhibitor Y-27632 (Sigma Aldrich) as described (Sato et al, 2009).
GLP-1 secretion has been investigated in vitro using a variety of cellular models, including fetal rat intestinal cell cultures, canine intestinal cell cultures enriched for L-cells by elutriation, and cell lines such as GLUTag, STC-1 and NCI-H716.
14 Intestinal epithelial cultures were isolated and cultured as described in Sato et al. 15 Colorectal tumour cell lines were maintained, and cell lysates prepared, as described in Emaduddin et al. 16 For de-novo methyltransferase inhibitor treatments, SW48 cells were plated in six-well plates at 2×10 per well.
