In B. subtilis, the gene encoding σK contains a 48 kb insertion sequence called the skin (sigma K intervening) element that must first be excised from the mother-cell chromosome by the recombinase SpoIVCA [ 79, 80].
This expression strategy was chosen because it has previously been demonstrated that the use of intervening IRES elements allowed bicistronic expression of the lux genes in the lower eukaryote Saccharomyces cerevisiae [19].
The presence of intervening IRES elements allows for transcription of a fused mRNA product from a single promoter, followed by cap-independent translation of the gene distal to the promoter concurrent with traditional cap-dependent expression of the promoter proximal gene [24].
The number of health professionals available is calculated using several data, including the number of health professionals in the baseline year (element 1) and the outflow of health professionals in the intervening years (element 8).
Thus, the present stimulus material with fixed syntactic frames containing 2 dependencies with 32 intervening X-elements, provides an interesting comparison to that used in the study by Gomez and colleagues [4], [11] which contained 3 dependencies with 24 intervening X-elements.
Therefore it appears that the riboswitches are still able to function as a regulatory structure, despite the intervening BOX element.
Moreover, this corrupted ycdT gene is separated from the pgaABCD operon by an intervening IS1 element (Fig 2B), which also disrupts the pgaA promoter sequence (as determined in E. coli K-12 by Wang et al (2005), with a K-12-like sequence confirmed here for W3110, the outbreak strain and HUSEC041).
It did not disrupt me, because disruption implies separation of activity, the intervening of outside elements.
Many subsystems and heterogeneous elements intervene.
"Yet, no single organization seemingly had the full perspective necessary to see and follow the family's progress, and intervene when these elements of self-sufficiency began to destabilize".
