The medium was aspirated at 24-hour intervals, transferred immediately to prelabeled micro-centrifuge tubes on ice, and then frozen prior to subsequent determination of the glucose and lactate concentrations, as below.
The microcosms were serially renewed at four-day intervals by transferring 3 ml aliquots to new microcosms containing 27 ml of fresh bacterial culture medium.
To characterize the interaction between DSiaT and β4GalNAcTA in more detail, we assayed the "kinetics" of paralysis by counting the number of paralyzed flies in a 3-min interval after transferring them to the restrictive temperature (38°).
Following sampler recovery, cores were sliced at one centimetre vertical intervals and transferred to buffered formaldehyde, diluted to 4% v/v with seawater.
Exactly 0.5 ml of each suspension was withdrawn at the appropriate time intervals and transferred to 4.5 ml nutrient broth (Oxoid Ltd) recovery medium containing 3% "Tween 80" to neutralize the effects of the antimicrobial compounds carry-over from the test suspensions.
New robust lag, lag-lead, PI controller design methods for control systems with a fractional order interval transfer function (FOITF) are proposed using classical design methods with the Bode envelopes of the FOITF.
Examples are given to show how the proposed method can be used to assess the effects of parametric variations on the stability in feedback loops with fractional order interval transfer functions.
Electric pulses (poring pulse: 75 V, 5 ms of length with 50-ms interval, transfer pulse: 20 V, 50 ms of length with 50-ms interval) were delivered using NEPA21 (Nepa Gene, Ichikawa, Japan).
After transferring intervals of 1,024 ms (1,024 data points) into the frequency domain using spectral analysis, the vibration frequency and three harmonic frequencies were cut out with a band width of 5 Hz.
The calcium-scoring CT was reconstructed using a window centered at 75% of the RR interval and transferred to commercially available software (Intelli Space Portal ver. 5; Philips Electronics Japan, Tokyo) to calculate the Agatston score.
