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Aliquots were withdrawn at the given time intervals, heated in a boiling water bath for 3 min, 0.5 mM EDTA was added and centrifuged at 10,000g for 10 min.
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For the two well locations we calculated mean heat flow values from the interval heat flow values.
The sample was heated at 2 °C intervals within 300 s equilibration time.
To maintain a correct water temperature (approximately outer skin temperature, 35°C), heating foil is placed in the lower section of the floating tank, regulated by a thermostat and heated in regular intervals.
Microwave on medium for 2 minutes, then stir and continue heating in 1 minute intervals until the potatoes are heated through.
Plants were heat shocked at 37°C for 2 h and stained for GUS activity at various intervals following heat shock, to monitor the rate of GUS turnover.
The enzyme (1.0 μg) was further heated for various time intervals at 45°C.
E0 and Et are original activity and residual activity after being heated for different time intervals.
The enzyme sample was heated at 58°C for various time intervals.
The solution was then heated in domestic microwave for variable time intervals.
Interval surface heat flow (qinterval) and mean surface heat flow (qmean) are given in mW m−2.
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