Measurement of flow rate through the tube was accomplished via collection of outflow blood over timed intervals.
Separation/elution of peptides was achieved using an in-line capillary C-18 column (Magic C-18, Micron Bioresource) applying a gradient from 5 to 95% acetonitrile in 0.1% formic acid over a 35 min time interval at a flow rate of 250 nl/min.
The 33-mer, 26-mer and fragments thereof were eluted using a linear gradient from 0% to 55% buffer B containing 80% (v/v) acetonitrile and 0.1% (v/v) TFA over a 75 min time interval at a flow rate of 1.0 ml/min [19].
Analytes were separated using a 38.5 min gradient of 25 52% 50 mM ammonium formate, pH 4.5, against acetonitrile over a 38.5 min interval with a flow rate of 0.4 mL/min.
A total of 110 fractions were collected at 5 min intervals at a flow rate of 0.75 ml min−1.
The personal breathing zone samples were collected throughout the workshift during 120-minute intervals with a flow rate of 0.1 liters/minute.
The results showed that, at a 95% confidence interval, the effect of air flow rate was relatively insignificant, while the effects of feed total solids, feed flow rate and inlet air temperature were at the same significance level.
Then 100 300 ml preformed blood clot was injected until systemic circulation ceased (systolic AP < 25 mmHg) and a 5 minute interval was allowed before start of CPB (flow rate of 4 7 l/min).
One of the objectives in this research was to evaluate the effect of mass flow rate and interval time of crop discharging on the rate of crop drying by the dryer.
According to Duncan's multiple range test, the effect of mass flow rate of drying air, and the interval time of discharge on rate of drying, were significant at 0.01 level.
