Cleavage was carried out in the reaction buffer finally optimized for the intertwined action of reaction conditions, resulting in synergistic maximum TaqII affinity star activity (40 mM Tris HCl, pH 8.0, at 65°C, 10 mM MgCl2, 10 mM (NH4 2SO4, 1 mM DTT, BSA 100 μg/ml, 100 μM SIN, 20% DMSO) at 65°C.
Israel's attorney general, Yehuda Weinstein, said Thursday in a report announcing his decision that he could not adequately prove a link between Mr. Lieberman and the money, though he said, "The suspicions against Lieberman's series of intricate and intertwined, underhanded actions cannot be ruled out".
Network structure and individual agency are intertwined: structure determines actions, which subsequently shape the structure (Bourdieu 1977; Gulati and Gargiulo 1999).
The difference between the two intertwined frames of action is clear and simple: Beatty, as writer and director, is sympathetic to Marla and Frank but uninterested in them, whereas he's deeply invested, both as a filmmaker and as a performer, in Hughes.
We examine the intertwined nature of the actions of endotoxin and fungal components, sphingolipid biology in PKD, the structure of PKD gene products, infections, and integrity of gut function to establish a mechanistic hypothesis for microbial provocation of human cystic disease.
But as years go by, and people slowly get accustomed to buying and using and transferring them, and as Facebook grows more and more intertwined with every online action we take which is to say, nearly every action we take it could well become the first global virtual currency … and then ultimately lose that "virtual" disclaimer.
Issues of miRNA target detection and miRNA mode of action are intertwined with discussion of the major effect and roles of miRNAs in the cell.
We also used mathematical modeling to support our experimental results, and this modeling suggests that the different types of drug-tolerant cells we have seen result from the actions of two intertwined switches, one which involves oxygen-based metabolic switching.
(Soccer club chair, man, 31) Also potentially intervening between knowledge and action were the intertwined emotional, social and gender dynamics framing sexual experience and behaviour.
Because the strategic themes or action areas are intertwined and complementary in practice, it was difficult to classify any child protection intervention under a single strategic category.
