Sentence examples for interruption of the gene from inspiring English sources

We have generated heparanase deficient mice through targeted interruption of the gene in ES cells.

This, coupled with a potential ribosome binding site and secondary translational initiation codon, suggests that the NMA2121 homologue may be expressed in N. gonorrhoeae strain FA19, despite the apparent interruption of the gene by a CREE.

However, because the flies carried the mutated genes from birth, the preferences researchers saw in these studies could have been due to abnormal brain development rather than an interruption of the genes' action in the adult nervous system.

This is aggravated by polymorphisms, among others due to the frequent interruption of the genes in the main repeats by repetitive elements such as the ones found early on in D. melanogaster ([ 36, 37]) and other insects [ 38], including A. gambiae[ 39]; these insertions usually, but not exclusively, interrupt the 28S gene.

Modification of essential genes is also problematic using the two-step procedure because the first step results in interruption of the target gene by the mutation cassette.

We have also demonstrated that interruption of the katE gene has a marked effect on catalase activity in growth-arrested cells.

To confirm complete interruption of the Hpse gene, we examined the expression of heparanase mRNA derived from different tissues of wt and Hpse-KO mice by real time PCR using specific primers as summarized in Table 1.

To determine what effect interruption of the hfq gene has on the F. novicida phenotype, two separate transposon insertion mutants were obtained from a F. novicida library created by Gallagher and co-workers [37].

This may unmask recessive alleles and generate phenotypes not linked to the interruption of the target gene.

Surprisingly, this unique HS-specific glycosidase is not essential for animal development and homeostasis, as demonstrated by targeted interruption of the heparanase gene in mouse [ 24].

From two independent transfection experiments, the analysis of genomic DNA obtained from resistant stable parasites by PCR (from 2 months up to 9 months of culture under blasticidin pressure), with specific primers indicated in Additional file 1: Table S1, did not evidence the interruption of the PfI2 gene.