Interrogation and clinical data examination were performed to each patient to collect the following information: age, weight, height, body mass index (BMI), comorbidities, age at onset of menopause, hormonal replacement therapy, family history of fractures, personal history of fractures and their incidence condition, current treatment and calcium dietary intake.
The first data interrogation was performed prior to any intervention, and reflected baseline antimicrobial prescribing habits.
The second data interrogation was performed during a period of active antibiotic stewardship promotion.
The third data interrogation was performed following the addition of a care bundle to the prescribing module of Care Vue.
In light of the presence of compact network substructures, further functional interrogation was performed by decomposing the networks into tightly linked clusters of genes.
In the remainder, notifications occurred on the day of travel to the hospital where a face-to-face interrogation was performed.
Sensor interrogation is performed using an external cavity tunable diode laser centered at 1550 nm coupled via fiber optics to a free-space optical scanner.
DNA isolation, amplification and SNP chip interrogation was performed using the method previously developed in house [ 80], with several alterations to the manufacturer's instructions, including 2 punches being removed as opposed to 1, heating of the cards and washes being performed using water instead of FTA purification reagent and Tris EDTA.
