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But its interrogations function is less well known, even to close observers of Chicago police.
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A specific TAR capture/expression vector can be assembled with sequence information for the target region of genomic DNA, facilitating the in vivo interrogation of function for captured genes.
Large-scale screening in Drosophila cultured or primary cells facilitates rapid genome-scale interrogation of gene function.
Very recently, two studies further expanded the application of CRISPR/Cas9 to genome-wide interrogation of gene function through systematic transcriptional perturbation.
The 'finished' reference genome sequence of rice has been available to the community now for a decade, serving as nucleator for hypothesis-driven interrogation of gene function and evolution.
To enable systematic protein function interrogation in a multicellular context, we built a genome-scale transgenic platform for in vivo expression of fluorescent- and affinity-tagged proteins in Caenorhabditis elegans under endogenous cis regulatory control.
As an optical approach, it is inherently scalable for remote and parallel interrogation of biological function at the tissue level; with implantable miniaturized devices, the technique is uniquely suitable for in vivo tracking of function, as illustrated by numerous applications in the brain.
Interrogation of DMC1 function revealed a level of redundancy with known RAD51 activities.
High-throughput cell-based RNA interference (RNAi) screening, including in Drosophila cultured cells, provides a format for large-scale interrogation of gene function.
Our approach enabled both facile production of the alterochromides and in vivo interrogation of gene function associated with alterochromide's unusual brominated lipid side chain.
Studies in Drosophila are favored by the availability of an extensive genetic toolkit that enables genome-wide, muscle-specific interrogation of gene function during normal aging and in response to pharmacological, dietary and environmental interventions.
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