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In addition to this observation and explanation, we can further deduce that an increase of sampling window size at the right image proportion will improve tracking accuracy due to capture of the required features at appropriate interrogation space.
In brief, labeled antibodies were detected with the ZeptX system, in which liquid from each well is pumped through an interrogation space within a capillary flow cell.
When fluorescently-labeled antibodies pass through the interrogation space, they emit light that is measured using a confocal microscope lens and a photon detector.
The trade-off is the loss of any information on spatial distribution of fluorescence lifetime within the cells and the limited number of photons obtainable from each cell due to their finite dwell time in the interrogation space.
Laser light (wavelength ∼650 nm) is directed into the interrogation space, and the resulting emission from each labeled antibody (wavelength 668 nm) is measured via a confocal microscope with a photon detector.
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We can digitize the analytic velocity field over a Cartesian grid with each coordinate denoted by (x,y), and with velocity interrogation spacing, δ to produce a velocity vector flow field, ux,y at a resolution of δ/L.
Various radiation transport simulations pertaining to active and passive interrogation, medical physics, space applications, and other disciplines involve the motion of objects.
SOLiD sequencing is based on two-base interrogation during color-space sequencing, which retains all four sequencing colors, even when sequencing a genome lacking several of the 16 possible two-base combinations, such as in bisulfite sequencing (Figure 3).
Screams echoed around the open space from interrogation rooms above.
The specification and interrogation of the model space is an outstanding conceptual issue for DCM.
A clear window above the interrogation region allowed free-space detection.
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