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Understanding cellular processes occurring in vivo on time scales of days to weeks requires repeatedly interrogating the same tissue without perturbing homeostasis.
You don't need to duplicate your address book, or even synchronize it: whether you're looking for a number to call from the Android phone app, or looking for an email from the Ubuntu email app, both programs are interrogating the same single address book.
Although redundant probe sets are expected to be consistent if they are interrogating the same transcript in a data set, random sampling will always introduce some variation.
The consistency of the P/A calls between probe sets interrogating the same gene provides an indicator of how differently the redundant probe sets from the same gene behave at this level.
Arrays interrogating the same CNV region in the same individual would be expected to yield inconsistent results if the probes are in different locations; in one case they identify copy number changes while in the other they may not.
It has been demonstrated that thermodynamic models of hybridization taking into account such sequence-dependent effects are capable to significantly reduce signal fluctuation between probes interrogating the same target [3] [6].
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These numbers allow the inclusion of multiple probes to interrogate the same target sequence, providing statistical rigor to data interpretation.
One document, "Statements of citizens of Kaliningrad, collected by myself", revealed that Kuchumov had even interrogated the same witness, Feyerabend.
U.S. intelligence agencies sometimes interrogate the same detainees the HIG questions -- and claim better results.
For Panahi, though, the act of stealing stands in opposition to the act of filmmaking: To steal something is to affirm its reality, whereas to film a thing is to interrogate the same.
This approach does not require that allele frequency be calculated from a large number of individually genotyped samples and does not assume that probes interrogate the same SNP with different 25 base oligos having the same hybridization properties.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com