Therefore, we interrogated the expression of WEE1 in publicly available datasets that detail the expression profiles of human breast tumour cell lines and tumours [22], [23].
To interrogate the expression of PPAR-γ target genes in the dataset, 87 genes known from the literature to be regulated by PPAR-γ were extracted from the microarray data.
The cRNA was then fragmented and hybridized to HG-U133A GeneChip arrays (Affymetrix, Santa Clara, CA), which contain 22,283 probe sets designed to interrogate the expression of more than 13,000 unique human genes.
Two color microarrays experiments were performed by using Agilent platforms; 45,220 probe sets were tested to interrogate the expression of 30,886 unique human genes.
This array was used to interrogate the expression of 9,238 known genes in duodenum tissue of four resistant and four susceptible female lambs.
The expression level of a single mRNA, defined as the signal, was determined by the MAS 5.0 software which uses a weighted average fluorescence intensity difference obtained among the 11 probe pairs that interrogate the expression of each individual gene.
This shows that the arrays have sufficient species specificity to interrogate the expression of a considerable fraction of genes in fused cells, but it also demonstrates the need for validation by more stringent methods.
To examine the molecular events associated with the homeostatic response to UCB treatment, high density oligonucleotide microarrays (Affymetrix GeneChip Human U133A 2.0) were used to interrogate the expression of over 14,500 transcripts.
Exploiting the high conservation of coding sequences in mammals, we used a human microarray platform to interrogate the expression of the donor human and recipient hamster genes in the G3 RH panel.
As a first step to validate functionally a role for ABCC5 in promoting bone metastasis, we interrogated the expression of ABCC5 in breast cancer cell lines with high bone-metastatic potential.
