Recently, chip-based methods [ 22- 32] (and references [ 26- 29]) such as Affymetrix gene expression arrays have been used for SNP discovery Affymetrix short oligonucleotide arrays are fabricated with sets of eleven 25-mer probes to interrogate the expression level of a particular mRNA.
Comprehensive gene expression profiles of 9 independent healthy donors were generated with high-density oligonucleotide human arrays with 22,283 probe sets, which in total interrogated the expression levels of approximately 18,400 transcripts and variants, including 14,500 well-characterized human genes.
We interrogated the expression levels of TRAIL and its receptors in our pre-existing microarray data sets on the above oral cell lines representing different phenotypes.
Comprehensive gene expression profiles for the three attenuated and the three virulent M. bovis macrophage infections were generated with high-density oligonucleotide bovine arrays (Affymetrix) and 24,072 probe sets, which in total interrogated the expression levels of approximately 23,000 transcripts.
Having confirmed that the reciprocal F1 triploids generated from reciprocal interploidy crosses were truly isogenic, the tiling arrays were used to interrogate the expression levels of 25 703 genes in seedlings.
High throughput genomic technologies, by simultaneously interrogating the expression levels of thousands of genes, offers the potential to identify new biomarkers for early detection, prognosis, targets for therapy and for reclassification of prostate tumors.
The expression level of a single mRNA, defined as the signal, was determined by the MAS 5.0 software which uses a weighted average fluorescence intensity difference obtained among the 11 probe pairs that interrogate the expression of each individual gene.
Therefore, we interrogated the expression of WEE1 in publicly available datasets that detail the expression profiles of human breast tumour cell lines and tumours [22], [23].
Efforts have been made into exploring effective strategies to interrogate the expression and activation of kinases.
We also interrogated the expression pattern of several splicing activators, again, no obvious pattern was found.
